Ability to biofilm formation in infectious agents isolated from patients of a large general children’s hospital

Author:

Savilov E. D.1ORCID,Markova Y. A.2ORCID,Nemchenko U. M.3ORCID,Noskova O. A.4,Chemezova N. N.1ORCID,Kungurtseva E. A.3ORCID,Dukhanina A. V.3

Affiliation:

1. Scientific Centre of the Family Health and Human Reproduction Problems; Irkutsk State Medical Academy of Continuing Education

2. Siberian Institute of Physiology and Biochemistry of Plants

3. Scientific Centre of the Family Health and Human Reproduction Problems

4. Scientific Centre of the Family Health and Human Reproduction Problems; Irkutsk State Regional Children’s Hospital

Abstract

Objective: The study objective is to analyze biofilm formation in microorganisms isolated from patients of children’s hospital with severe purulent septic diseases.Methods: The ability to biofilm formation was studied in microorganisms isolated from 32 patients aged from 1 to 15 y.o. with sepsis, acute hematogenous osteomyelitis, peritonitis, and pneumonia in intensive care unit of regional general children’s hospital. Blood, phlegm, bronchial and oropharyngeal washings, wound fluid, peritoneal fluid served as specimens.Results: All tested strains have the ability to biofilm formation; moreover, the majority of them formed moderate biofilms.Conclusion: The common spread of biofilm infections can be related to the most important factors of preservation and distribution of microorganisms in health facilities which significantly limit preventive and therapeutic measures. 

Publisher

Pacific State Medical University

Subject

General Medicine

Reference13 articles.

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2. The national concept of prevention of the infections connected with delivery of health care, 2011. (In Russ). URL: http://www.garant.ru/products/ipo/prime/doc/70000121/ (Accessed Apr 24, 2019).

3. Anganova EV, Savilov ED, Ushkareva OA, Ablov AM, Duhanina AV. Ability of pathogenic and opportunistic enterobakteriya to formation of biofilms. Acta Biomedica Scientifica. 2014;5:34–7 (In Russ).

4. Yarets Yu, Shauchenka N. A new method for the bacterial biofilms analysis in medicine. Science and Innovations. 2016;10: 64–68 (In Russ).

5. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol. 1985;22(6):996–1006.

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