Diversity, Distribution and DNA Barcoding Tribe of Anomalini beetles (Coleoptera: Scarabaeidae: Rutelinae) in Wheat Fields of Van, Türkiye

Abstract

Anomalini beetles (Coleoptera: Scarabaeidae: Rutelinae) constitute a significant pest group responsible for substantial crop losses in wheat cultivation areas worldwide, including Turkey. This study aimed to comprehensively evaluate the phylogeny, diversity, abundance, and distribution of Anomalini beetles in wheat fields of Van province, Turkey. Surveys were conducted between April and August 2021, involving monthly sample collection at predetermined locations within six districts: Başkale, Çaldıran, Erciş, Gevaş, İpekyolu, and Tuşba. Standard traps and hand collection methods were employed to gather samples. In molecular studies, the mitochondrial COI gene region has been amplified and sequenced using universal primers. Anomalini beetles were detected in all sampling areas except Çatak district. Seven species were identified: Anisoplia austriaca, A. signata, A. lata, Brancoplia leucaspis, Blitopertha nigripennis, Chaetopteroplia segetum, and an unidentified Anisoplia sp. Chaetopteroplia segetum emerged as the most prevalent and abundant species across all districts. Notably, all identified Anomalini species represent the first records for Van province and its environs. While Anomalini beetles were present in the region, their population densities were not considered high enough to cause economic damage.