The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle

Abstract

As the global population grows, there is a need to produce higher yields in food, agriculture, and livestock. To achieve this, scientists are exploring new techniques and methods. However, it is crucial to select the right candidate genes and markers, especially in techniques like QTL and MAS in livestock, to ensure success. We conducted a study to determine allele frequencies and their association with milk yield in 70 Simmental cattle breeds in two lactations using the PCR-RFLP technique. The statistical analysis was conducted using the general linear model procedure with the least square method The study focused on the allele frequencies for the POU1F1-HinfI gene in Simmental cattle. The dominant B allele frequency for POU1F1/Hinf1 was 0.58, while the A allele frequency was 0.42. Simmental population was under HardyWeinberg Equilibrium (HWE) for the POU1F1-HinfI genotypes (p>0.05) The study found that genotype frequencies were in balance for POU1F1/Hinf1. No significant correlation between POU1F1-HinfI gene polymorphisms and milk yield was found, but they have been associated with growth and reproductive traits in various cattle breeds. The results could provide useful information for breeding programs aimed at improving the performance traits of Simmental cattle.