Development and Detection of Antimicrobial Properties of Polyherbal Handwash

Abstract

Many of the medications marketed as traditional herbal medicines have unquestionably been used for a very long time. Hands have always been the dominant source of transmission of infection to patients. Thus, encouraging "personal hygiene" is the main objective of developing a herbal hand wash. The current study's goal was to develop a formulation of polyherbal handwash employing methanolic extracts of dried leaves of Azadirachta indica, Ocimum gratissimum, and Coriandrum sativum. Other ingredients entailed lemon juice, aloe vera, lavender oil, HPMC, triethanolamine, sodium lauryl sulphate (SLS), glycerine, and methylparaben. Four batches of hand wash formulations were prepared, and each batch was tested for stability, appearance, colour, grittiness, pH, viscosity, foam height, and other physical characteristics. Using the agar well diffusion method, the anti-microbial effectiveness of the prepared polyherbal hand wash was tested on a variety of bacteria, including Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeroginosa, Salmonella typhi, Aspergillus niger, and Candida albicans. The results demonstrated that manufactured herbal handwash formulations, particularly F3, displayed a prominent zone of inhibition in comparison to standard commercial handwash, indicating that the extract of these phytoconstituents may be employed to manufacture handwash with antimicrobial properties. As a result, the research shows that the herbal handwash formulation is analogous to commercial handwash in reducing the amount of bacteria on hands and may be used as a replacement handwash made from natural sources without experiencing any undesirable effects.