An Approach for Developing a Simple and Quick Method for Separation of Asiatic Acid and Asiaticoside Rich Fraction From Centella Asiatica and Simultaneous Determination by Reversed-Phase High-Performance Liquid Chromatography

Abstract

In Asian countries, Centella asiatica is exploited for abundant types of pharmacological activities due to the presence of opulent phytochemicals. Asiaticoside, madecassoside, and their sapogenin triterpene acids such as asiatic acid and madecassic acid are the most noticeable triterpenes present in C. asiatica. To date, numerous techniques/methods are used to extract and isolate the different kinds of phytoconstituents from C. asiatica. Still, most methods require some special requirements, and some procedures are monotonous and time-consuming. Meanwhile, previously reported methods used for the extraction and isolation were not validated for large-scale production, yield, and purity. The study's primary goal is to develop the methodology for extracting and isolating the Asiaticoside and asiatic acid from C. asiatica at the minimum time with the highest yield and purity. Asiaticoside and asiatic acid extraction and isolation involved the acid hydrolysis method and recovered in alcohol. The Reverse Phase-High Performace Liquid Chromatography (RP-HPLC) method was developed and validated as per ICH guidelines for quantifying both compounds. The obtained results indicate that the developed method produces asiaticoside and asiatic acid with good purity. As per the ICH guidelines, the RP-HPLC was developed and validated. The proposed method can be used to isolate the asiaticoside and asiatic acid from C. asiatica. Some modification in this method leads to the large-scale production of highly pure asiatic acid and asiaticoside for their versatile application in the area of cosmetics and phytopharmaceuticals.