Investigation of Hemoglobin Conformation Changes in Patients with Pulmonary Hypertension under the Action of Alternating Magnetic Field

Abstract

The influence of magnetic field on human health is still not fully understood. The effects of short-term and long-term effects of both, strong and weak electric and magnetic fields are being actively studied. One of the main areas of research is the study of the effect of magnetic fields on the blood and on individual blood components, in particular the hemoglobin molecule. The purpose of this research work is to study of short term (1, 15 and 30 minutes) influence of 300 mT alternating magnetic field (AMF), which is equivalent to that observed from conventional sources of statistical magnetic field, on isolated hemoglobin, as well as on hemoglobin in erythrocytes of blood samples of donors with pulmonary hypertension before and after treatment with Raman spectroscopy. The effect of AMF on both the conformation of heme (iron-containing site) and its protein part (globin) has been established, hence the effect on the ability of hemoglobin to bind ligands, in particular oxygen. Change in the level of oxygenated and deoxygenated hemoglobin and an increase in the probability of finding hemoglobin in T-form were revealed. A change in the polarity of the environment of amino acid residues was also revealed. There was a decrease in the polarity of the environment of amino acid residues, as well as the ordering and density of amino acids. In experiments with erythrocytes, a significant increase in cell membrane fluidity was observed.