STRESS-PROTECTIVE PROPERTIES OF THE 1,2,4-TRIAZOLE-3-THIOL DERIVATIVES COMPOUND UNDER ACUTE STRESS CONDITIONS OF LAYING HENS

Abstract

A new synthesized substance codenamed ASP-34 was used as the research object. The stress-protective properties were studied during prophylactic oral consumption of the compound under conditions of 4-hour acute heat and immobilization stress of laying hens in comparison to the control groups and the reference substance with proven stress-protective, hepatoprotective and immunostimulating effects «Cyanophor» (cyanocobalamin+butaphosphane). In serum and liver homogenate, the content of markers of the antioxidant-prooxidant system was determined: the quantitative content of diene conjugates (DC), thiobarbituric acid reactants (TBA-reactants), as well as the activity of catalase and superoxide dismutase (SOD). The results of the study demonstrate the presence of probable stress-protective properties of the 1,2,4-triazole-3-thiol derivative compound ASP-34 on the model of acute 4-hour heat and immobilization stress of laying hens. The test sample compound of the 1,2,4-triazole-3-thiol derivative ASP-34 in this study contributed to the restoration of the levels of TBA-reactants and DC (both in the serum and in the liver parenchyma) to the levels of similar indicators in negative control group (p>0.05 vs. NC). Moreover, there was also a tendency to increase the serum activity of SOD by 2.4 times (p=0.09) from the values of this indicator in the positive control group. According to the results of the biochemical study, it is clear that both of the tested agents in the indicated doses showed an antioxidant effect against the background of acute thermal and immobilization stress of chickens, but no significant difference between the activity of the two agents was noted. The practical value of the obtained results is the confirmation of the stress-protective properties of the compound of the 1,2,4-triazole-3-thiol derivative ASP-34 on the model of acute 4-hour heat and immobilization stress of laying hens and the following recommendation for conducting tests in production conditions.