JUSTIFICATION OF CANDIDATE POLYMORPHISMS USAGE IN MARKER-ASSISTED SELECTION OF UKRAINIAN MEATY PIG BREED

Abstract

Effective selection in pig breeding is not possible without involvement of new approaches which involve the assessment animal genotypes at the DNA level. The development of methods for determining the polymorphisms in candidate genes that are responsible for the manifestation of economic traits is the basis of modern marker selection technology (MAS). Currently, a number of DNA markers have been developed for use in the breeding of farm animals. In this case, the most informative were single nucleotide polymorphisms (SNPs) of genes. However, despite the significant amount of scientific research, the problem of development and implementation of DNA markers for breeds of Ukrainian selection remains relevant. The results of SNPs study of RYR1, CTSF and CTSD genes by PCR-RFLP method are presented. The initial stage for implementing marker selection for single nucleotide polymorphisms is conducting genetic-population analysis in the studied population of Ukrainian Meaty pig breed. It was found that SNP RYR1 g. 1843 C>T was characterized by low polymorphism, the minor allele g. 1843 T met with a frequency of q = 0.05. SNP CTSD g. 70 G>A had a low level of representativeness, allele g. 70 A prevailed at a frequency of q = 0.92. It was found that SNP CTSF g. 22 G>C was characterized by a sufficient level of representativeness, both alleles were detected with a predominance of the allele frequency g. 22 G (q=0.80). In the population of Ukrainian Meaty pig breed, there was a statistically confirmed deviation of genotype frequencies from SNP-balanced CTSF g. 22 G> C (χ2 = 28.125) and CTSD g. 70 G> A (χ2 = 26,518). In the future, SNPs of CTSF, CTSD genes can be used for associative studies to find a link between markers and signs of pig productivity and the introduction of marker-associated selection in the UM pig breed.