BIOMARKERS OF LIPID AND PROTEIN OXIDATION IN THE MUSCLE TISSUE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) TREATED IN VITRO WITH ROOT AND STEM EXTRACTS OF GREATER CELANDINE (CHELIDONIUM MAJUS L.)-Reference-Cited by-同舟云学术

BIOMARKERS OF LIPID AND PROTEIN OXIDATION IN THE MUSCLE TISSUE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) TREATED IN VITRO WITH ROOT AND STEM EXTRACTS OF GREATER CELANDINE (CHELIDONIUM MAJUS L.)

Published:2024 Issue:131 Volume: Page:202-214
ISSN:2312-8402
Container-title:The Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine
language:
Short-container-title:STBIAS

Author:

Tiupova Tetiana¹^ORCID,Tkaczenko Halina¹^ORCID,Kurhaluk Natalia¹^ORCID,Lukash Oleksandr²^ORCID

Affiliation:

1. Institute of Biology, Pomeranian University in Słupsk, Poland

2. T.H. Shevchenko National University “Chernihiv Colehium”, Chernihiv, Ukraine

Abstract

The main aim of the present study was to evaluate the oxidative stress biomarkers [TBARS, carbonyl derivatives of oxidative modification of proteins (OMP), total antioxidant capacity (TAC)] in the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) after in vitro incubation with the root and stem extracts derived from greater celandine (Chelidonium majus L., CM) (at final concentrations of 5 and 2.5 mg/mL) collected in South Park in Słupsk in the Pomeranian Province (northern part of Poland). The current study demonstrated the increase in TBARS levels after in vitro incubation of rainbow trout muscle tissue with stem and root extracts of CM at a final concentration of 5 mg∙mL-1 compared to untreated control samples. There was a statistically significant increase in TBARS levels compared to controls. We obtained similar results after in vitro incubation with root and stem extracts of CM at a final concentration of 2.5 mg∙mL-1 with rainbow trout muscle tissue, where we also observed a statistically non-significant increase in TBARS levels. There was a decrease in the levels of aldehydic derivatives and ketonic derivatives of OMP in muscle tissue after incubation with extracts from roots and stems of CM at final concentrations of 5 mg∙mL-1 compared to untreated controls. There was a decrease in the levels of aldehydic and ketonic derivatives of OMP in muscle tissue after incubation with extracts from roots and stems of CM at final concentrations of 2.5 mg∙mL-1 compared to untreated controls. TAC levels in rainbow trout muscle tissue after in vitro incubation with extracts of CM roots and stems at final concentrations of 5 and 2.5 mg∙mL-1 were not statistically significantly increased. The present study investigated the antioxidant potential of CM. Extracts from CM roots and stems exert their activity by inhibiting protein damage.

Publisher

Institute of Animal Science of the National Academy of Agrarian Sciences of Ukraine

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