A simple and effective method for inactivating virus infectivity in formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease

Author:

Brown Paul,Wolff Axel,Gajdusek D. Carleton

Abstract

We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Neurology (clinical)

Cited by 123 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3