GAMMA-RAYS AND MICROWAVES IRRADIATION INFLUENCE ON GUAR (CYAMOPSIS TETRAGONOLOBA): II – PROTEOMIC ANALYSIS LINKED TO PLANT HEIGHT AND CRUDE PROTEINS

Abstract

Guar is an economically significant forage crop and a multi-purpose plant. In Egypt, with a limited gene pool of guar, gamma ray (γ-rays) and microwave pre-treatment, individually or in association, were used to generate genetic variability and develop new high-yielding genotypes. Guar seeds of the variety Shandaweel-9 were irradiated with different γ-ray doses (i.e., 0, 150, 250, and 350 Gray “Gy”), either individually or along with the irradiation with 900 W microwave treatments applied for different exposure times (1, 2, 3, and 4 min), then grown at Agricultural Research Center, Giza, Egypt in 2019, and 2020 summer seasons. The results indicated that gamma-ray doses of 150 and 250 Gy, stand-alone or with microwave treatments, significantly influenced guar plant height and crude protein in M1 and M2. Fragments with 925, 1427, and 2145 bp linked to plant height and crude protein were eluted from the gel, sequenced, and then registered on GenBank. The fragment linked to plant height received accession No. LC681484.1 and fragments linked to crude protein received accession No. of OK617330.1 and OK617331.1. The sequenced fragments underwent translation to protein, then proceeded to proteome analyses. Depending on the sequence analysis, the 925 bp fragment consisted of a 302 bp ORF (open reading frame) encoded with 302 amino acids. However, the fragment of 1427 bp has 433 ORF, and the fragment of 2145 bp has 705 ORF. The LC681484.1 might have a role in plant elongation. On the other hand, OK617330.1 and OK617331.1 might be responsible for cell wall protein organization.