Effect of glutathione administration as adjuvant therapy to NO and MDA level in Wistar rat peritonitis model

Abstract

Background: Excessive amounts of free radicals beyond the antioxidant levels in the body will cause a condition called oxidative stress. Nitric oxide (NO) and malondialdehyde (MDA) are produced under conditions of oxidative stress. Glutathione (GSH) is the most endogenous antioxidant synthesized in humans. Therefore this study aims to prove whether the administration of glutathione as an adjuvant therapy can reduce levels of NO and MDA in peritonitis.Methods: This is an experimental study with a post-test-only control group design. Twenty-four Wistar rats were randomly divided into four groups (6 rats/group). The first group (C) was a control group. The second group (P) was induced by peritonitis. The third group (P+Cef) was peritonitis-induced groups with given Ceftriaxone injection as therapy. The fourth group (P+Cef+Glu) was peritonitis-induced groups with given Ceftriaxone injection and glutathione as adjuvant therapy. After 24 hours, blood samples were taken for examination of NO and MDA levels. Data is considered significant if the p-value <0.05.Results: There was a significant increase in NO level from Group 2 to Group 4 (P<0,05). There were no significant differences between MDA levels groups (P>0,05).Conclusion: Glutathione as adjuvant therapy is not adequate to reduce NO and MDA levels in peritonitis.