Morphological and Biochemical Investigation of the Healing Effects of Exercise on High Fat Diet Induced Kidney and Bladder Damage

Abstract

Objective: The aim of this study was to assess the preventive effects of swimming exercise on kidney and bladder damage caused by a high-fat diet (HFD) using morphological and biochemical measures. Methods: Sprague Dawley rats were fed either standard chow (CONT, 6% fat) or HFD (45% fat) for 18 weeks, these rats were divided into two subgroups at the last 6 weeks of the experiment. The exercise groups (CONT+EXC, HFD+EXC) were trained daily swimming sessions (1 h per day for 5 days/week) during the last 6 weeks. Kidney and bladder samples were prepared for light and electron microscopic examination at the end of experiment. Malondialdehyde, glutathione, interleukin-6, and tumor necrosis factor-α were measured by biochemically.Results: Regular morphology of renal cortex and urinary bladder mucosa were observed in the CONT and CONT+EXC groups. Degenerated renal corpuscles and proximal tubules in kidney and degenerated urothelium with leaky tight junctions and increase of mast cells in bladder mucosa were observed in the HFD group. Ameliorated renal cortex and bladder mucosa were observed in the HFD+EXC group. Moreover, malondialdehyde, glutathione, interleukin-6 and tumor necrosis factor- α levels were compatible with histological findings. Conclusion: HFD induced kidney and bladder damage may have linked to increased oxidative damage. It was observed that histological damage and altered oxidative stress parameters were reversed with swimming exercise, and it is thought that moderate swimming exercise may have a role in the regulation of oxidative stress.