In Vitro Assessment of Anti-inflammatory Effect of Apigenin on Renal Cell Inflammation

Abstract

Objective: The aim of this study was to evaluate in vitro effect of apigenin on anti-inflammatory cytokines interleukin (IL)-10 and transforming growth factor beta (TGF-β) levels in an in vitro model of renal cell inflammation induced with lipopolysaccharide (LPS). Methods: Renal cell inflammation was formed in the African green monkey kidney cell line (Vero). Four groups as NC (negative control group, any application was not done), LPS (treatment with 10 μg/ml of lipopolysaccharide during 4 hours), API (treatment with 5 μg/ml of apigenin during 12 hours) and LPS+API (treatment with 10 μg/ml of lipopolysaccharide during 4 hours+5 μg/ml of apigenin during 12 hours) were formed. Cytotoxic effect of apigenin in Vero cells was evaluated by cell count test. Inflammation dose of lipopolysaccharide was determined by measuring Tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations in cell culture supernatants. TNF-α, IL-6, IL-10 and TGF-β concentrations in cell culture medium were measured by ELISA using ELISA test kits. Results: IL-6 and TNF-α concentrations of LPS group increased compared to NC, API and LPS+API groups (p