Chemical Constituents and Anti-Neuroblastoma Activity from Boesenbergia stenophylla

Abstract

Three diarylheptanoids and one flavonoid, i.e. 7-(4-hydroxy-3-methoxyphenyl)-1-phenylhept-4-en-3-one (4), 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenylheptan-3-one (5), 1,7-diphenylhept-4-en-3-one (6), and 3,5,7-trihydroxyflavone (7) were isolated and characterized from the rhizome of Boesenbergia stenophylla. Compounds 2 and 4 displayed excellent anti-neuroblastoma activity which reduces the cell viability to 30% and 20%, respectively. The results from the molecular docking experiments targeting the protein kinases regulating neuroblastoma cell survival (PI3K/AKT1 signalling pathway) are consistent with that of the in vitro results. Finally, the structures of 4-7 were elucidated using spectroscopic methods (UV, IR, NMR, and HRESIMS).