Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
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Published:2023-02-14
Issue:1
Volume:48
Page:28-36
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ISSN:2460-6278
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Container-title:Journal of the Indonesian Tropical Animal Agriculture
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language:
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Short-container-title:J. Indonesian Trop. Anim. Agric.
Author:
Setiatin E. T.1,
Lestari D. A.1,
Harjanti D. W.1,
Setiaji A.1,
Kurnianto E.1
Affiliation:
1. Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
Abstract
The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Publisher
Institute of Research and Community Services Diponegoro University (LPPM UNDIP)
Subject
General Veterinary,Animal Science and Zoology