Association between leptin gene polymorphism and growth traits in Bali cattle

Author:

Kurlyana T.1,Hartatik T.2,Sumadi S.3

Affiliation:

1. Graduate Student at Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia

2. Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia

3. 2Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia

Abstract

Leptin (LEP) gene produces hormone leptin which is secreted by adipose tissue, and plays an im-portant role in energy balance, regulating feed intake, regulating endocrine function, and immune functions. This study aims to identify molecular markers of LEP gene and its association with growth traits based on SNP in Bali cattle. The blood samples were collected from 16 male and 30 female Bali cattle. The growth data were recorded from 2018 to 2020, consist of body weight and body size. Am-plification of leptin gene with polymerase chain reaction (PCR) using pair of primers, Lep-tin_3Forward: 5’- AGCTTGGAAACATGGTGGTC-3’ and Leptin_3Reverse: 5’- CATGATGCTCCCTGGATTCT-3’ with DNA target 898 bp. The SNPs were identified by the direct sequencing technique. Genotypes of the SNPs were identified using sequencing method. Association of LEP genotypes with growth traits was performed using oneway ANOVA. Three DNA polymor-phisms of the LEP gene were found, namely g.2913C/T, g.3260T/C, and g.3549G/A. SNP g.2913C/T was significantly associated (P<0,05) with weaning shoulder height (WSH), weaning body length (WBL), weaning chest circumference (WCC), yearling shoulder height (YSH), yearling body length (YBL), and yearling chest circumference (YCC). Meanwhile, SNPs g.3260T/C and g.3549G/A were not associated with the body weight and body size in Bali cattle. In conclusion, the SNP g.2913C/T can be used as molecular marker for body size in weaning and yearling of Bali Cattle.

Funder

Indonesia Endowment Fund for Education

Publisher

Institute of Research and Community Services Diponegoro University (LPPM UNDIP)

Subject

General Veterinary,Animal Science and Zoology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Polymorphisms of the Leptin gene in Jabres cattle;IOP Conference Series: Earth and Environmental Science;2024-05-01

2. MILK PRODUCTIVITY AND MILK QUALITY OF COWS WITH DIFFERENT LEP GENOTYPES AND LINEAR AFFILIATION;Agrobiotechnologies and digital farming;2023-10-24

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