Antibacterial Activity and Mechanism of Action of Methanol Extract from Kasturi Mango Fruit (Mangifera casturi) on Caries-Causing Bacterium Streptococcus mutans

Author:

Suhendar Usep1,Fathurrahman Muhammad2,Sogandi Sogandi3ORCID

Affiliation:

1. Faculty of Pharmacy, Pakuan University Bogor

2. Chemistry Department, Faculty of Mathematics and Natural Sciences, Pakuan University Bogor

3. Universitas 17 Agustus 1945 Jakarta

Abstract

One of the problems frequently found in the oral cavity is dental caries caused by Streptococcus mutans. Thus far, dental caries is treated using antibiotics. However, the bacterium is known to be resistant to many antibiotics; hence, another alternative is needed. An alternative option is found in the Kasturi mango (Mangifera casturi). This study aims to identify the bioactive compounds of  Kasturi mango and find out the mechanism of its action in inhibiting the growth of Streptococcus mutans. Kasturi mangoes were macerated using 96% methanol, then the phytochemical compounds were identified qualitatively. Antibacterial activity testing was carried out using the agar diffusion method, and bioactive compounds were identified using GCMS. The results showed that the methanol extract of Kasturi mango contains alkaloids, flavonoids, phenolics, terpenoids, and saponins. In 1 gr/mL of the methanol extract of Kasturi mango fruit has an inhibitory activity against the growth of Streptococcus mutans with a zone of inhibition of ± 10 mm and MIC (minimum inhibitory concentration) value of 25% extract. The inhibitory action is suspected to be through a mechanism where holes in the bacterial cell membrane are made. This can be seen from the results of SEM (scanning electron microscope) images showing that cell leakage or lysis has occurred. This research also, for the first time, revealed the types of bioactive compounds from the methanol extracts of Kasturi mango (Mangifera casturi) consisting of 18 compounds with the most abundance is 5-Hydroxymethylfurfural compounds, Octadecenoic acid, n-Hexadecanoic acid, Phenyl 4-methyl-1-piperidine carboxylate, and Methyl linolenate.

Publisher

Institute of Research and Community Services Diponegoro University (LPPM UNDIP)

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3