Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil

Author:

Camboim Expedito K. A.1,Almeida Arthur P.1,Tadra-Sfeir Michelle Z.2,Junior Felício G.1,Andrade Paulo P.3,McSweeney Chris S.4,Melo Marcia A.1,Riet-Correa Franklin1

Affiliation:

1. Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande, 58708 Patos, PB, Brazil

2. Laboratório de Fixação Biológica de Nitrogênio, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, 80060 Curitiba, PR, Brazil

3. Departamento de Genética, Universidade Federal de Pernambuco, 50670 Recife, PE, Brazil

4. CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia

Abstract

The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats. The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture. The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L1sodium fluoroacetate (SF), incubated at 39°C in an orbital shaker.Pseudomonas fluorescens(strain DSM 8341) was used as positive control for fluoroacetate dehalogenase activity. Two isolates were identified by 16S rRNA gene sequencing asPigmentiphaga kullae(ECPB08) andAncylobacter dichloromethanicus(ECPB09). These bacteria degraded sodium fluoroacetate, releasing 20 mmol L1of fluoride ion after 32 hours of incubation in Brunner medium containing 20 mmol L1of SF. There are no previous reports of fluoroacetate dehalogenase activity forP. kullaeandA. dichloromethanicus. Control measures to prevent plant intoxication, including use of fences, herbicides, or other methods of eliminating poisonous plants, have been unsuccessful to avoid poisoning by fluoroacetate containing plants in Brazil. In this way,P. kullaeandA. dichloromethanicusmay be used to colonize the rumen of susceptible animals to avoid intoxication by fluoroacetate containing plants.

Funder

INCT for The Control of Plant Poisonings

Publisher

Hindawi Limited

Subject

General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine

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