Affiliation:
1. Laboratory of Biopharmaceuticals, Division of Cancer Research, Regional Cancer Centre, Trivandrum 695 011, India
2. INFORMM, Universiti Sains Malaysia, Health Campus 16150 Kubang Kerian, Malaysia
Abstract
Antitumor activity of polysaccharide PST001 isolated from the seed kernel ofTamarindus indicawas evaluated using different cancer cell lines. Human cancer cell lines A549, KB, and MCF-7 and murine cancer cell lines DLA and EAC were treated with PST001 and cell growth inhibition was assessed by MTT assay. In vivo studies were carried out for toxicity, tumor reduction and immunomodulation. The respective IC50of PST001 in A549, KB, and DLA was at 80.72, 190.99, and 91.14 μg/mL. Significant tumor reduction was obtained in both DLA and EAC tumors on treatment with PST001 which was more prominent when PST001 was administered with CTX/5-fluorouracil. Increase in total WBC, CD4+T-cell population, and bone marrow cellularity suggested strong immunomodulatory activity for this compound. No significant abnormality was observed in toxicity studies. Thus the results of the present study suggest that PST001 has immunomodulatory and tumor inhibitory activities and has the potential to be developed as an anticancer agent and immunomodulator either as a sole agent or as an adjuvant to other chemotherapeutic drugs.
Funder
Kerala State Council for Science, Technology and Environment
Subject
General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
56 articles.
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