Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry-Reference-Cited by-同舟云学术

Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

Published:2012 Issue: Volume:2012 Page:1-8
ISSN:1537-744X
Container-title:The Scientific World Journal
language:en
Short-container-title:The Scientific World Journal

Author:

Mikulíková Kateřina¹,Romanov Oleg²,Miksik Ivan¹³,Eckhardt Adam¹³,Pataridis Statis¹,Sedláková Pavla¹

Affiliation:

1. Institute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech Republic

2. Kalmykian State University, Elista, Russia

3. Cardiovascular Research Centre, Prague, Czech Republic

Abstract

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.

Funder

Grantová Agentura Ceské Republiky

Publisher

Hindawi Limited

Subject

General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/tswj/2012/759604.pdf

Reference32 articles.

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