Molecular Detection and Characterization of Goat Isolate ofTaenia hydatigenain Turkey

Author:

Utuk Armagan Erdem1,Piskin Fatma Cigdem1

Affiliation:

1. Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020 Ankara, Turkey

Abstract

The aim of this study was to provide molecular detection and characterization of the goat isolate ofTaenia hydatigenafrom Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified asTaeniaspp., and partial sequence of mt-CO1 gene was corresponding toT. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate ofT. hydatigenawas submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization ofT. hydatigenawas done for the first time in Turkey.

Publisher

Hindawi Limited

Subject

General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine

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