Wound Healing Gel Based on Chlorella vulgaris Extract Characterization

Abstract

Chlorella vulgaris cell extract was used to develop wound-healing products by our research group. However, it is required to characterize this formulated for improved patient compliance and to reduce the force required to spread the product on the injured, painful and sensitive areas. Therefore, C. vulgaris extract was characterized by the presence of phytochemicals and proteins, the formulation was characterized in relation to organoleptic properties, pH, and rheological profiles, while both cell extract and excipients of the formulation were analyzed by infrared spectroscopy and thermogravimetric (TGA). In the cell, extract contain 1.35 ± 0.18 mg/g AG of total phenol content and 65.57 μg mL-1 of one lectin of 36 kDa with the hemagglutinating activity of ≥ 23 for erythrocyte type B. The formulation showed a green color, smooth texture, pH of 7.09 ± 0.07, suitable for skin injury and pseudoplastic behavior. The infrared showed the presence of amine, carbohydrates, and terpenes in the cell extract and the absence of chemical interaction between the cell extract and carbopol hydrogel. The formulation is very stable near room temperature. The results obtained will provide parameters for the production of inputs in the pharmaceutical industry, as well as for the quality control of intermediates and the finished product based on C. vulgaris.