In Silico Analysis of Tumor Necrosis Factor (TNF) in Homo sapiens

Abstract

Tumor Necrosis Factor (TNF) encodes a pleiotropic proinflammatory cytokine that plays a major role in regulating immune cells. The cytokine involved the perception of a wide spectrum of biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It has also been implicated in various diseases such as autoimmune disorders, insulin resistance, cancer, etc. Considering the in-depth significance of TNF, the present study was designed for their in silico analysis in Homo sapiens. This study is undertaken to find the functional and structural basis of TNF in H. sapiens, by using various bioinformatics tools. In silico evaluation of these proteins includes physicochemical parameters, prediction of secondary structures, and validation of different models. These proteins could be directly or indirectly stabilized by the amino acid interactions and hydrogen-bond networks. It elucidated the functional deviations of protein to some extent and laid the foundation for docking and molecular modeling for future prospects.