Potential Inhibition of Ehrlich Ascites Carcinoma by Naja Nubiae Crude Venom in Swiss Albino Mice

Abstract

Cancer, the uncontrolled growth of cells, is a noteworthy cause of death globally. The present investigation attempted to study the potential efficacy of the Naja nubiae snake venom (NNSV) against Ehrlich Ascites Carcinoma (EAC) bearing mice. The LD50 was determined using twenty female mice (5 per group). The groups were given saline (Control) or venom (0.2, 0.46, 0.6 mg/kg) by intraperitoneal route. For the main experiment, fifty female mice were divided into five groups (n = 10): all groups except groups I and II received EAC cells intraperitoneal. All EAC bearing mice received intraperitoneal saline (EAC control), 0.034 mg/kg NNSV, 0.017 mg/kg NNSV, and reference drug (5- fluorouracil, 20 mg/kg body weight i.p.), respectively. In NNSV-treated mice, there were significant reductions in tumor volume, tumor cell counts, tumor cell viability, total WBC count, MDA, urea, uric acid, AST, ALT, and ALP levels. Red blood cell count, hemoglobin content, platelets, glutathione, and catalase levels increased significantly in NNSV-treated mice. The architecture of the hepatic and renal architecture in mice treated with NNSV was improved histopathologically. The effect of NNSV against Ehrlich Ascites Carcinoma was inversely dose-dependent.