Investigating the Effect of Loading Curcuminoids Using PCL-PU-βCD Nano-Composites on Physico-Chemical Properties, In-Vitro Release, and Ex-Vivo Breast Cancer Cell-Line

Abstract

Recently, cancer is deemed the main reason for death. Although most anti-cancer drugs have potent anti-cancer activity, their applications are greatly obstructed due to their flimsy solubility, stability, and high toxicity. Nanotechnology is considered the major distinctive tool for achieving highly safe and effective drug-delivery carriers. This survey studied the synthesis of poly-caprolactone polyurethane β-cyclodextrin (PCL-PU-βCD) amphiphilic copolymer and using such polymer with synthesized C5-curcuminoids as drug type to prepare polymeric-nano micelles by following two modified techniques, with confirming the synthesis of C5-curcuminoids, PCL, and PCL-PU-βCD, and the formation of polymeric-nanocomposites plus studying the formed nanocomposites; encapsulations, release patterns and kinetics, morphologies, physico-chemical properties and finally evaluating the death efficacy of these Curcuminoids-PCL-PU-βCD polymeric-nanocomposites on cancer cell cultures. The research results showed a successfully prepared C5-curcuminoid drug, PCL-PU-βCD polymer and Curcuminoids-PCL-PU-βCD polymeric-nanocomposites revealing; adequate encapsulations, controlled sustained releasing, promising physico-chemical properties, low cytotoxicity, and an auspicious IC50% on breast tumor cell-culture that were higher for the second preparation technique nanocomposites comparatively to the first preparation technique, despite such variation, both preparation techniques resulted in variances that were not significantly different (p>0.05).