UV-Vis Spectrophotometry Observation to Find Appropriate Wavelength for Non-Invasive Blood Haemoglobin Level Measurement Optical Device

Abstract

The wavelength convention for non-invasive blood hemoglobin measurement remains inconclusive. This experimental observation to find the appropriate wavelength candidate of LED for non-invasive blood hemoglobin level measurement optical device in 200 nm to 900 nm range. This observation ran in Prodia Kedoya and Biochemistry Lab, Universitas Krida Wacana, West Jakarta, in July 2019. The blood samples were obtained from 10 randomly selected consenting non blinded, healthy adult subjects between 18 and 60 years old. Each blood sample was diluted using double distilled water and measured absorbance using UV-Vis Spectrophotometry. Then it compared to blood hemoglobin level by standard gold measurement from Prodia Kedoya. The result shows functional group found in human Hb is C=O. Appropriate wavelengths were obtained based on the Pearson correlation, standard deviation, and human skin pigment, which are 525 nm, 550 nm, and 570 nm. Then the measurement of Hb levels is carried out at the selected wavelength and processed using a ZunZunSite3 to get the mapping data of Hb level from ten respondents. Root mean square error from the measurement. The error obtained is minimal, which indicates that the wavelength used is suitable for measuring the Hb level. Ethical Clearance: 076/IT3.KEPMSM-IPB/SK/2018.