Antioxidant Effect of the Complex Action of Vitamin E and Ethylthiosulfаnylate in the Liver and Kidneys of Rats under Conditions of Chrome(VI)-Induced Oxidative Stress

Abstract

The aim of our study was to investigate the efficacy and benefits of the complex effect of vitamin E and ethylthiosulfanylate (ETS) on the state of the pro/antioxidant system in the liver and kidneys of rats under the condition of Cr(VI)-induced oxidative stress. Rats were divided into 8 groups. Groups received: I (control) - physiological solution (150 μl) for 7 days; II – oil solution (1 ml) for 14 days; III, IV, VII, VIII - K2Cr2O7 (2.5 mg Cr(VI)/kg body weight (b.w)) for 7 (III, IV) and for 14 (VII, VIII); V - vitamin E (20 mg/kg b.w) for 14 days; VI, VII, VIII - vitamin E in complex with ETS (100 mg/kg b.w) for 14 days. Results report that K2Cr2O7 caused Cr(VI)-induced oxidative stress due to activation of lipid peroxidation (LP) processes. Cr(VI) action for 7 days caused compensatory activation of the antioxidant defense system (AOS) in both tissues. However, the longer action of Cr(VI) was accompanied by depletion of AOS enzyme activity and GSH content. The complex effect of vitamin E and ETS reduced the intensity of Cr(VI)-induced oxidative stress in both rat tissues. Our results indicate about positive antioxidant properties of vitamin E and ETS under the condition of Cr(VI) toxicity.