Cryoprotective Effect of Pyrano-[2,3-C]-Pyrazoles on H2O2 Induced Damage in Tetrahymena thermophila

Abstract

Antioxidants are developed to assist the immune system and overcome oxidative stress, break the auto-oxidative chain reaction, quench oxygen singlets (O2-), and inhibit peroxidase formation. The main objective of this study was to search for potent antioxidant supplements to protect our cells against diseases associated with oxidative stress. We chose four pyrano-[2,3-c]-pyrazole derivatives to test their antioxidant properties in vivo using "Tetrahymena spp" as a cellular model. Then, we measured the activity of some antioxidant and biochemical biomarkers such as CAT, SOD, GAPDH, SDH, Gr, and MDA. Also, the fragmentation of DNA has been investigated. The current study demonstrated that the three compounds (5a, b, and c) have potent antioxidant activity, characterized by increased activity of some antioxidant enzymes, inhibiting lipid peroxidation and DNA damage. These findings suggest for the first time that Pyrano-[2,3-c]-Pyrazoles is a promising source of synthetic antioxidants that could offer protection against H2O2-induced- stress and provide us with a new challenge to design a library of pharmaceutical compounds with high activity and low toxicity.