Development of Nanoemulsion containing Centella Asiatica Crude Extract as a Promising Drug Delivery System for Epilepsy Treatment

Author:

Abstract

Preparations of products containing herbal extracts have grown by leaps and bounds, hitting the pharmaceutical industries due to the natural healing approach. Centella asiatica L. (pegaga) (C. asiatica) is a famous plant commonly served as a salad in Asian diets. It contains various phytoconstituents, which plays a vital role in the treatment of various illness. For example, in the treatment of epilepsy, crossing the blood-brain barrier (BBB) has been a challenge (even as parenteral application) as not all the drugs were able to pass through the membrane and produce a maximum therapeutic effect to the targeted site of action. Thus, a nanoemulsion formulation containing C. asiatica crude extract needs to be developed to penetrate the BBB. This study aims to formulate a nanoemulsion containing crude extract of C. asiatica leaves for epilepsy treatment. Nanoemulsion was prepared by using low energy emulsification method. The particle size, polydispersity index (PDI), and zeta potential of C. asiatica crude extract nanoemulsion were found to be at 57.86 ± 0.03 nm, 0.50 ± 0.03, and -26.50 ± 0.03 mV, respectively. The formulation remained physically stable at different storage temperatures (4, 25, and 45 °C) for 90 days. The particle size observed by transmission electron microscopy (TEM) was shown to be at ̴50 nm, which correlated well with the Zetasizer analysis. The cytotoxicity study, conducted on formulated C. asiatica nanoemulsion towards Vero cell line and 3T3 cell line, showed that the IC50 value indicated that it is nontoxic (>500 µg/ml). C. asiatica nanoemulsion was found to be stable based on the good evidence of physicochemical properties, and the IC50 value indicates significance for future in vivo and in vitro studies based upon the route of administration.

Publisher

AMG Transcend Association

Subject

Molecular Biology,Molecular Medicine,Biochemistry,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3