Evaluation of Effectiveness of Covalently Immobilized α-Amylase and Lipase in Cleaning of Historical Textiles

Abstract

Historical textiles are exposed to damage as a result of many factors, such as climate changes. Cleaning of these historical textiles is very important. It can be done using the immobilization technology of enzymes. For this purpose, amylase and lipase were covalently immobilized on the surface of chemically activated alginate/carrageenan gel beads. Preparation, activation, and immobilization steps were characterized by FT-IR and TGA. Optimization of loading units and time for two enzymes were done. From the obtained results, we found that the optimum units and time for amylase are 25 U and 4 hrs, respectively. And the optimum units and time for lipase are15 U and 3 hrs, respectively. From the obtained results, immobilized α–Amylase and lipase can be used in the cleaning and restoration of historical textiles.