Screening for Probiotic Potential of Lactobacillus Rhamnosus Strain CRD4

Abstract

Lactic acid bacteria are the foremost used probiotic worldwide. Its successful application in manufacturing dairy products and probiotic foods makes it a promising industrial prerequisite. The objective of the present investigation was to isolate, identify, and molecularly characterize Lactic acid bacteria from local dairy samples of Odisha state of India and explores its probiotic traits. One potential strain was isolated using a selective Lactobacillus-MRS agar medium. The biochemical studies illustrated the bacteria were gram-positive, catalyze (-ve), and non-motile. The taxonomical diversity of the bacterium was analyzed by 16s RNA sequencing and classified as Lactobacillus rhamnosus strain CRD4 with gene bank accession no [MG573074]. Further, the selected strain was screened for its probiotic competence of lower acid and bile tolerance. The result confirmed that lactobacillus strain successfully defended the low pH and bile stress and acclaimed 70% cell surface hydrophobicity. Antibiotic studies obtained confirmed the possible resistance of the strain. The maximum zone of inhibition was expressed in diameter 42mm against Ciprofloxacin. In conclusion, based upon the above results, Lactobacillus rhamnosus can be a profound probiotic candidate.