The Effect of Myrtus, Honey, Aloe vera and Pseudomonas Phage Treatment on Infected Second Degree Burns: in vivo Study

Abstract

Many researches have been exerted to find an application dressing for wound healing and also attain a considerable microbial reduction in burn wounds. In this study, the healing effect of a mixed herbal ointment (containing Myrtus, honey, Aloe vera, and pseudomonas phage) on the healing process of second degree burn wounds infected with Pseudomonas aeruginosa in comparison was evaluated. For this purpose, a hot metal square piece (4×2 cm, 50 g) was applied using a standard burning technique, and the applied pressure on the skin kept the same for all animals, then infected with Pseudomonas aeruginosa. Rats were randomly divided into 2 groups. Group 1 was treated with mixed herbal ointment, and group 2 received no treatment (control group). The treatment was daily, and sampling was weekly for three consecutive weeks (7, 14, and 21-day). Formalin 10% was used for tissue fixation. Wound healing in test and control groups was investigated by macroscopic and microscopic methods using Hematoxylin-Eosin staining. It wound contraction evaluation (Image J software). Macroscopic findings showed that wound contraction of the mixed herbal ointment group was significantly higher than the control group for 21 days. Hematoxylin-Eosin staining revealed that the epithelialization was considerably more completed in the mixed herbal ointment group in comparison with the control group. Also, neovascularization was significantly higher in the mixed herbal ointment group. The comparative results demonstrated that the mixed herbal ointment group had a significant difference (P<0.05) with a non-treated group (control). Therefore, the mixed herbal ointment is suggested as a suitable candidate for the treatment of second-degree burn wounds infected to pseudomonas aeruginosa.