Graphene Oxide as a Carrier for Drug Delivery of Methotrexate

Abstract

Nanomaterials, including carbon-based nanoparticles, have been applied as carriers for anticancer drugs. This short communication reported the synthesis, cytotoxicity, and drug delivery of methotrexate using graphene oxide (GO) as a carrier. GO was synthesized following Hummer's method. It was characterized using X-ray diffraction (XRD), transmission electron microscope (TEM), and scanning electron microscope (SEM). Data analysis confirms the synthesis of GO with high crystallinity and lamellae morphology. GO showed a high cytocompatibility toward the human EA.hy926 endothelial cells. GO has been used as a carrier for the anticancer drug; methotrexate. The drug delivery was tested for hepatocellular carcinoma cells (HepG2 cells), human embryonic kidney cells (HEK293A cells), and porcine skin fibroblasts (PEF).