Assessment of neutrophil NETs‑forming activity in patients with ulcerative colitis

Abstract

The aim of the study. To determine the non-tossing capacity of neutrophils in patients with ulcerative colitis (UC).Materials and methods. Ten patients with UC (study group) were included in the study; the control group consisted of 22 healthy volunteers of comparable age to the study group. All patients with UC received treatment according to clinical guidelines. We determined the non-tumorforming ability of the isolated neutrophil fraction in vitro in both groups using an original technique developed by members of the authors' team (Invention Patent No. 2768152 C 1; dated 23.03.2022. Application No. 2021129097 dated 06.10.2021).Results. In patients with UC, after 15 minutes of in vitro stimulation with probiotic, there was a statistically significant increase in early netosis cells (p < 0.010), cloud-like traps (p < 0.001) and filamentous NVLs (p < 0.001) compared with the control group. Bacterial uptake rate by neutrophil traps in UC patients was statistically significantly lower than in controls (p < 0.010).Conclusions. All identified features of neutrophils non-trapping capacity in UC patients may indicate changes in granulocyte reactivity, probably, under the influence of cytokines and/or due to therapeutic intervention. The neutrophil traps formed had fewer protective effects and probably more damaging effects. Studying the influence of the role of netosis on the course and prognosis of UC is a promising research area.