Activation of lymphocytes in vitro for immunotherapy of patients with melanoma-Reference-Cited by-同舟云学术

Activation of lymphocytes in vitro for immunotherapy of patients with melanoma

Published:2021-12-06 Issue:31 Volume:1 Page:13-18
ISSN:2078-5631
Container-title:Medical alphabet
language:
Short-container-title:Medicinskij alfavit

Author:

Abakushina E. V.¹^ORCID,Gel’m Yu. V.²^ORCID,Lyssuk E. Yu.³^ORCID

Affiliation:

1. Medical Radiological Research Center n.a. A. F. Tsyb – a branch of Federal Medical and Biological Centre ‘National Medical Research Centre for Radiology;Obninsk Institute for Nuclear Power Engineering – National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)

2. Medical Radiological Research Center n.a. A. F. Tsyb – a branch of Federal Medical and Biological Centre ‘National Medical Research Centre for Radiology

3. National Medical Research Centre for Pediatric Hematology, Oncology and Immunology n.a. Dmitry Rogachyov

Abstract

This article describes a method for activating lymphocytes isolated from the peripheral blood of melanoma patients and cultured in a medium supplemented with IL‑2 and IL‑15. It was shown that in these terms, lymphocytes have an increased proliferative and activation potential. The combination of cytokines has a positive effect on cytotoxicity, viability and the expression of activation markers (CD38, CD69, CD25, HLA-DR and NKG2D) on NK- and T-lymphocyte, and may be recommended for the culture of lymphocytes in melanoma patients for the purpose of adoptive immunotherapy.

Publisher

Alfmed LLC

Subject

Materials Chemistry,Economics and Econometrics,Media Technology,Forestry

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