Author:
Guselnicova V V,Polevschikov A V
Abstract
Background. Studying of thymic mast cells population in normal state and after stressinduced atrophy. Methods. The study was performed on 80 thymus of white outbred mice with using of histochemistry and immunohistochemistry methods. Sections of embryonic thymus were stained with toluidine blue. Adult mice were given a single injection of 2,5 mg of hydrocortisone for induction of thymic accidental transformation; sections were stained with toluidine blue and alcian blue-safranin. Within of immunohistochemical research paraffin sections of adult thymus were stained with polyclonal antibodies to synaptophysin and tyrosine hydroxylase with alcian blue stain. Results. Mast cells (MCs) appeared in thymus on 19th day of embryonic life and demonstrated mainly a medullar location. In adult animals MCs were observed only in the connective tissue of the capsule, interlobular septa, subcortex and perivascular space. Mast cells matured in thymus after accidental transformation. The localization of developing mast cells was changing from medullar and cortical to capsular. A morphological proximity between nerve terminals and mast cells has been observed in normal adult thymus. Some of these nerves were catecholaminergic. Conclusion. Possible important role of thymic mast cells and mast cellsnerves interaction in normal state and after accidental transformation is discussed.
Reference22 articles.
1. Гусельникова В.В., Пронина А.П., Назаров П.Г, Полевщиков А.В. Происхождение тучных клеток: современное состояние проблемы. Вопросы морфологии XXI века. 2010, вып. 2, с. 108-115.
2. Arinobu Y., Iwasaki H., Gurish M. et al. Developmental checkpoints of basophil/mast cell lineages in adult murine hematopoiesis. PNAS. 2005, v. 102, p. 18105-18110.
3. Jamur M.C., Grodzki A.C., Berenstein E.H. et al. Identification and characterization of undifferentiated mast cells in mouse bone marrow. Blood. 2005, v. 105, p. 4282-4289.
4. Combs J.W., Lagunoff D., Benditt E.P. Differentiation and proliferation of embryonic mast cells of the rat. J. Cell Biol. 1965, v. 5, p. 577-592.
5. Ribatti D., Crivellato E. Mast Cells and TumoursTumors: from Biology to Clinic. Springer. New York. 2011, 142 p.