Author:
Raupach Michael J.,Hannig Karsten,Morinière Jérôme,Hendrich Lars
Abstract
The genus Amara Bonelli, 1810 is a very speciose and taxonomically difficult genus of the Carabidae. The identification of many of the species is accomplished with considerable difficulty, in particular for females and immature stages. In this study the effectiveness of DNA barcoding, the most popular method for molecular species identification, was examined to discriminate various species of this genus from Central Europe. DNA barcodes from 690 individuals and 47 species were analysed, including sequences from previous studies and more than 350 newly generated DNA barcodes. Our analysis revealed unique BINs for 38 species (81%). Interspecific K2P distances below 2.2% were found for three species pairs and one species trio, including haplotype sharing between Amaraalpina/Amaratorrida and Amaracommunis/Amaraconvexior/Amaramakolskii. This study represents another step in generating an extensive reference library of DNA barcodes for carabids, highly valuable bioindicators for characterizing disturbances in various habitats.
Funder
Bundesministerium für Bildung und Forschung
Subject
Animal Science and Zoology,Ecology, Evolution, Behavior and Systematics
Cited by
31 articles.
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