Antithrombotic Effect of Malayan Pit Viper Venom on Experimental Thrombosis of the Inferior Vena Cava Produced by a New Method

Abstract

A reliable method of producing thrombosis of the inferior vena cava in dogs is described. This consists of placing a segment of umbilical tape across the lumen of the vein and sewing it in place without interfering with blood flow. In the control animals gross thrombi were not usually present in the immediate postoperative period but were demonstrated after 24 hours. By the 7th postoperative day large red clots completely obstructing the vessel were usually found. The venom of the Malayan pit viper proved to be highly effective in preventing experimental thrombosis. The antithrombotic effect of viper venom is believed to be due primarily to induced hypofibrinogenemia. Accelerated fibrinolysis may also be a factor. The experiments suggest that Malayan pit viper venom may be effective as an antithrombotic agent.