Affiliation:
1. From the Department of Physiology, Faculty of Medicine, Université de Montréal and Institut de Cardiologie de Montréal (Québec), Canada.
Abstract
Background
We considered that β
2
-adrenergic stimulation may dilate resistance coronary vessels by opening ATP-sensitive potassium (K
ATP
) channels, thereby triggering NO formation.
Methods and Results
In conscious instrumented dogs after β
1
-adrenergic blockade, intracoronary (IC) injections of acetylcholine (ACh), nitroglycerin (NTG), and pirbuterol (PIR), a selective β
2
-adrenergic agonist, were performed before and after blockade of NO formation with IC
N
ω
-nitro-
l
-arginine methyl ester (L-NAME, 50 μg·kg
−1
·min
−1
×12 minutes) or blockade of K
ATP
channels with IC glibenclamide (25 μg·kg
−1
·min
−1
×12 minutes followed by 2 μg·kg
−1
·min
−1
). PIR (50.0 ng/kg) increased coronary blood flow (CBF) by 32±6 from 43±7 mL/min and by only 11±2 (
P
<.01) from 40±7 mL/min after L-NAME. Increases in CBF to ACh were also reduced by L-NAME, but NTG responses were not. Before glibenclamide, PIR increased CBF by 33±5 from 45±7 mL/min and by only 14±3 (
P
<.01) from 36±5 mL/min thereafter. CBF responses to ACh and NTG were maintained after glibenclamide. Lemakalim, a selective opener of K
ATP
channels, caused dose-dependent increases in CBF that were partially inhibited by L-NAME. In experiments in which CBF was controlled, the fall in distal coronary pressure caused by PIR was less after L-NAME or glibenclamide than before.
Conclusions
β
2
-Adrenergic dilation of resistance coronary vessels involves both the opening of K
ATP
channels and NO formation. L-NAME antagonized lemakalim responses consistent with a link between the opening of K
ATP
channels and NO formation in canine resistance coronary vessels.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine
Cited by
36 articles.
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