Candidate Genes in the Regulation of Na + Transport by Inner Medullary Collecting Duct Cells From Dahl Rats

Abstract

Abstract —Recently, we reported that primary cultures of inner medullary collecting duct cells from Dahl salt-sensitive (S) rats absorb more Na + than do cells cultured from Dahl salt-resistant (R) rats. To begin to evaluate the molecular basis for this difference, we selected four candidate gene products that on the basis of their physiology and genetics could participate in regulation of Na + transport by these cells. During 24-hour exposure, inhibitors of the cytochrome P450 enzymes had no effect on Na + transport by either S or R monolayers. Twenty-four-hour exposure to N G -monomethyl- l -arginine (0.5 mmol/L), a nonspecific inhibitor of NO synthase, also had no effect on Na + transport by either S or R monolayers. Neither atrial natriuretic peptide 1–28 (100 nmol/L) nor 8-Br-cyclic GMP (100 μmol/L) had any short-term effect on Na + transport by either S or R monolayers. 18-Hydroxy-11-deoxycorticosterone (100 nmol/L), an adrenocorticoid hormone that is produced in greater amounts in S rats, stimulated Na + transport by both S and R monolayers via the mineralocorticoid receptor; however, its effect was less potent than aldosterone. Congenic rats in which the R isoform of the 11β-hydroxylase gene was bred onto the S background had monolayers that transported Na + at a rate similar to the S rats. These results demonstrate that neither cytochrome P450 genes, NO synthase genes, the atrial natriuretic peptide receptor gene, nor the 11β-hydroxylase gene is a likely candidate to explain the difference in Na + transport between S and R inner medullary collecting duct monolayers in primary culture.