Affiliation:
1. From the Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, and the Department of Human Dry Dock, Sanraku Hospital (K.Y.), Tokyo, Japan.
Abstract
Abstract
A major biologically active Na,K-ATPase inhibitor in the mammalian body may be ouabain-like compound. We investigated the potential roles of circulating ouabain-like compound in the regulation of Na
+
and K
+
homeostasis in terms of Na
+
and K
+
distribution between the cells and the extracellular fluid (internal balance). First, we developed a population of rats immunized against ouabain to block the action of ouabain-like compound. We measured plasma and intracellular Na
+
and K
+
concentrations in skeletal muscle and determined Na
+
(extracellular-to-intracellular concentration ratio) and K
+
(intracellular-to-extracellular concentration ratio) gradients in immune rats. We examined also the ability to respond to hypertonic NaCl load in immune rats. Consistent lower plasma K
+
levels and steeper Na
+
and K
+
gradients were observed in immune rats. K
+
handling in response to hypertonic NaCl load was altered, and lower plasma K
+
level was maintained in immune rats. Second, we used PST-2238, a newly developed anti-ouabain agent, to block the action of ouabain-like compound and examined its effect on plasma Na
+
and K
+
concentrations. Chronic administration of PST-2238 significantly lowered plasma K
+
levels in rats with subtotal nephrectomy. These findings collectively suggest that ouabain-like compound may determine at least in part the internal Na
+
and K
+
distribution and the transmembrane cation gradients in vivo in rats.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Cited by
10 articles.
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