Isolation and characterization of single vascular smooth muscle cells from spontaneously hypertensive rats.

Author:

Bolzon B J1,Cheung D W1

Affiliation:

1. Department of Pharmacology, University of Toronto, Ontario, Canada.

Abstract

To study the properties of vascular smooth muscle in hypertension without the influence of the nerves and endothelium, a procedure was developed to isolate single smooth muscle cells from tail arteries of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) normotensive control rats. Perfusion of intact arteries with a solution of papain and collagenase produced dense populations of viable cells (more than 10(4) cells/ml) that remained relaxed in the presence of physiological levels of calcium. Contractile responses of smooth muscle cells from the SHR were significantly more sensitive to noradrenaline, potassium depolarization, and the calcium channel agonist Bay K 8644 compared with those from WKY rats. Enhanced sensitivity to calcium in the SHR was also observed on readdition of calcium to cells preincubated in noradrenaline or KCl in a calcium-free medium. These results provide evidence for alterations in the properties of vascular smooth muscle in the SHR at the single cell level.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Internal Medicine

Reference26 articles.

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2. Recent advances in the pathogenesis of hypertension: Consideration of structural, functional, and metabolic vascular abnormalities resulting in elevated arterial resistance

3. Cellular basis for increased sensitivity of vascular smooth muscle in spontaneously hypertensive rats;Hermsmeyer K;Ore Res,1976

4. Cocaine and contractile responses of vascular smooth muscle from spontaneously hypertensive rats;Webb RC;Arch Int Pharmacodyn,1981

5. Adrenergic neurotransmission in vascular smooth muscle from spontaneously hypertensive rats.

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