Affiliation:
1. Division of Vascular Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Abstract
Recent evidence suggests that endothelium-derived relaxing factor exhibits properties of nitric oxide. Like nitric oxide, it inhibits platelet function and mediates its effects by elevating intracellular cyclic GMP. In this study we have investigated the role of reduced thiol in the mechanism of action of endothelium-derived relaxing factor on platelets. Bovine aortic endothelial cells were grown on microcarrier beads and pretreated with aspirin before use. Endothelial cells stimulated with bradykinin or exposed to stirred medium expressed a dose-dependent inhibition of platelet aggregation that was potentiated by the reduced thiol, N-acetylcysteine. Endothelial cell-mediated platelet inhibition was attenuated by methylene blue. Inhibition of platelet aggregation by endothelial cells was associated with a rise in platelet intracellular cyclic GMP, an effect that was enhanced by N-acetylcysteine. These data show that 1) the reduced thiol N-acetylcysteine potentiates platelet inhibition by endothelium-derived relaxing factor and 2) this effect is associated with increasing intracellular platelet cyclic GMP levels.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine,Physiology
Reference55 articles.
1. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
2. Endotheliumderived relaxing factor from pulmonary artery and vein possesses pharmacologic and chemical properties identical to those of nitnc oxide radical;Ignarro LJ;Ore Res,1987
3. Interaction of nitrogen oxides, oxygen and amines in gaseous mixtures;Ncurath GB;IARC Sd Publ,1976
4. Superoxidc anions and hyperoxia inactivate endothelium-derived relaxing factor;Rubanyi GM;Am J Physiot,1986
5. Studies of endothelium-derived relaxant factor (EDRF), its nature and mode of action
Cited by
241 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献