Affiliation:
1. Department of Physiology, University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania 15213
Abstract
The in vitro calcium uptake activity has been examined in fragments of sarcoplasmic reticulum, i.e. vesicles, obtained from hearts spontaneously failing in the canine heart-lung preparation. The rate of uptake was found to be less than that observed for vesicles from control hearts. If the vesicles were prepared from a failed heart that had been treated in vivo with ouabain, then the rate of calcium uptake was normal. In vitro additions of ouabain increased the rate of calcium uptake only with vesicles prepared from failing hearts. This effect of ouabain on calcium uptake by vesicles appears, therefore, to be specific for the defect causing depression of calcium uptake. No evidence could be found that the depression of calcium uptake is due to uncoupling of the pump adenosine triphosphatase. Calcium uptake and adenosine triphosphatase activity were found to be proportionately depressed in the failing heart and proportionately restored by ouabain.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine,Physiology
Reference29 articles.
1. The significance of calcium ions in cardiac excitation and contraction
2. Metabolism of the heart in failure. In Symposium on Congestive Heart Failure, 2d ed., New York;FENTON J. C;American Heart Association,1966
3. Excitation-contraction coupling in skeletal muscle;SANDOW A.;Pharmacol. Rev.,1965
4. Drugs and Properties of Heart Muscle
5. Calcium uptake by cardiac vesicles: Inhibition by amytal and reversal by ouabain;BRICCS F. N.;Biochem. Z.,1966
Cited by
96 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献