A Technique for the Microscopic Study of Blood Vessels in Living Striated Muscle (Cremaster)

Author:

MAJNO GUIDO1,GILMORE VIRGINIA,LEVENTHAL MONIKA

Affiliation:

1. Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115

Abstract

A technique is described whereby it is possible to study the vessels of a striated muscle (the rat cremaster) in vivo. In the anesthetized rat, a part of the scrotal skin is excised; the cremaster muscle is exposed and studied by epi-illumination. To improve the optical conditions, a white reflecting surface is brought behind the cremaster through the inguinal canal. Twitching of the muscle may be prevented by the use of a neuromuscular blocking agent. It is also possible to study the blood vessels of the fascia overlying the cremaster. The method may also be applied to the rabbit; it is recommended for short-term studies in which topographic requirements prevail. Its advantages and limitations are discussed.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Cardiology and Cardiovascular Medicine,Physiology

Reference17 articles.

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2. Current trends in microcirculatory research;ZWEIFACH B.;Proceedings of theIX Microcirculatory Conference. Angiology,1961

3. Direct observation of skeletal muscle blood vessels (rat cremaster)

4. Studies of Vascular Patterns in Living Tumors With a Transparent Chamber Inserted in Hamster Cheek Pouch2

5. Cinephotomicroscopy of living tissues in medical research and teaching, insertion and use of a rabbit ear chamber for time-lapse and standard cinephotomicroscopy in the living animal;WOOD S.;J. Soc. Motion Picture Television Engrs.,1965

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