NaHCO 3 Dilates Mouse Afferent Arteriole Via Na + /HCO 3 − Cotransporters NBCs

Abstract

Sodium bicarbonate has long been used to treat chronic kidney disease. It has been demonstrated to slow the decline in glomerular filtration rate in chronic kidney disease patient; however, the mechanisms are not completely understood. We hypothesized that NaHCO 3 dilates afferent arterioles (Af-Art) by stimulating nitric oxide (NO) release mediated by the Na + /HCO 3 − cotransporter (NBC) contributing to the elevation in glomerular filtration rate. Isolated microperfused mouse renal Af-Art, preconstricted with norepinephrine (1 µmol/L), dilated 45±2% (n=6, P <0.05) in response to NaHCO 3 (44 mmol/L). Whereas, NaCl solution containing the same Na + concentration was not effective. The mRNA for NBCn1 and NBCe1 were detected in microdissected Af-Art using reverse transcription–polymerase chain reaction and quantitative polymerase chain reaction. The Af-Art intracellular pH measured with 2′,7′-bis-(2-carboxyethyl)-5-(and-6) carboxyfluorescein, acetoxymethyl ester increased significantly by 0.29±0.02 (n=6; P <0.05) in the presence of NaHCO 3 , which was blunted by N-cyanosulphonamide compound (S0859) that is an inhibitor of the NBC family. After clamping the intracellular pH with 10 μM nigericin, changing the bath solution pH from 7.4 to 7.8 still dilates the Af-Art by 53±4% (n=7; P <0.005) and increases NO generation by 22±3% (n=7; P <0.005). Both pH-induced NO generation and vasodilation were blocked by L-NG-Nitroarginine Methyl Ester. NaHCO 3 increased NO generation in Af-Art by 19±4% (n=5; P <0.005) and elevated glomerular filtration rate in conscious mice by 36% (233 versus 318 ul/min; n=9–10; P <0.0001). S0859 and L-NG-nitroarginine methyl ester blocked NaHCO 3 -induced increases in NO generation and vasodilation. We conclude that NBCn1 and NBCe1 are expressed in Af-Art and that NaHCO 3 dilates Af-Art via NBCs mediated by NO that increases the glomerular filtration rate.