Affiliation:
1. From the Department of Veterans Affairs Medical Center, Boston, Mass; the Department of Medicine, Boston University School of Medicine, Boston, Mass; the Weis Center for Research, Geisinger Clinic, Danville, Pa; and the Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Md.
Abstract
Abstract
The spontaneously hypertensive rat (SHR) exhibits a transition from stable compensated left ventricular (LV) hypertrophy to heart failure (HF) at a mean age of 21 months that is characterized by a decrease in α-myosin heavy chain (α-MHC) gene expression and increases in the expression of the atrial natriuretic factor (ANF), pro-α
1
(III) collagen, and transforming growth factor β
1
(TGF-β
1
) genes. We tested the hypotheses that angiotensin-converting enzyme inhibition (ACEI) in SHR would prevent and reverse HF-associated changes in gene expression when administered prior to and after the onset of HF, respectively. We also investigated the effect of ACEI on circulating and cardiac components of the renin-angiotensin system. ACEI (captopril 2 g/L in the drinking water) was initiated at 12, 18, and 21 months of age in SHR without HF and in SHR with HF. Results were compared with those of age-matched normortensive Wistar-Kyoto (WKY) rats, and to untreated SHR with and without evidence of HF. ACEI initiated prior to failure prevented the changes in α-MHC, ANF, pro-α
1
(III) collagen, and TGF-β
1
gene expression that are associated with the transition to HF. ACEI initiated after the onset of HF lowered levels of TGF-β
1
mRNA by 50% (
P
<.05) and elevated levels of α-MHC mRNA two- to threefold (
P
<.05). Circulating levels of renin and angiotensin I were elevated four- to sixfold by ACEI, but surprisingly, plasma levels of angiotensin II were not reduced. ACEI increased LV renin mRNA levels in WKY and SHR by two- to threefold but did not influence LV levels of angiotensinogen mRNA. The results suggest that the anti-HF benefits of ACEI in SHR may be mediated, at least in part, by effects on the expression of specific genes, including those encoding α-MHC, ANF, TGF-β
1
, pro-α
1
(III) collagen, and renin-angiotensin system components.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Cited by
43 articles.
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