Gene expression and atrial natriuretic factor processing and secretion in cultured AT-1 cardiac myocytes.

Author:

Lanson N A1,Glembotski C C1,Steinhelper M E1,Field L J1,Claycomb W C1

Affiliation:

1. Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112.

Abstract

BACKGROUND Studies were carried out to characterize several biochemical features of cultured AT-1 cells. METHODS AND RESULTS These cells were obtained from a transplantable atrial cardiomyocyte tumor lineage. Reverse transcriptase-polymerase chain reaction-based analyses demonstrated that the pattern of gene expression of cultured AT-1 cells was similar to that of adult atrial myocytes. AT-1 cells expressed atrial natriuretic factor (ANF), alpha-cardiac myosin heavy chain, alpha-cardiac actin, and connexin43. Radioimmunoassays verified that the cells synthesized, stored, and secreted ANF. Through size-exclusion, reversed-phase, and carboxymethyl-ion-exchange high-performance liquid chromatography, it was shown that cultured AT-1 cells stored ANF as pro-ANF (ANF-[1-126]), which was cosecretionally processed quantitatively to ANF-(1-98) and the bioactive 28-amino-acid ANF-(99-126). In addition, cultured AT-1 cells secreted ANF at almost a sixfold greater rate in response to endothelin-1, a potent secretagogue of ANF. KCl, metenkephalinamide, isoproterenol, phenylephrine, and 12-O-tetradecanoyl-phorbol-13-acetate also stimulated ANF release. CONCLUSIONS These studies, in combination with previous findings, demonstrated that cultured AT-1 cells, while maintaining the ability to proliferate, have retained functional, biochemical, and ultrastructural features that are characteristic of adult atrial myocytes.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine

Reference34 articles.

1. Atrial Natriuretic Factor-SV40 T Antigen Transgenes Produce Tumors and Cardiac Arrhythmias in Mice

2. Steinhelper ME Field LT: SV40 large T-antigen induces cardiocyte proliferation in transgenic mice in Oberpriller JO Oberpriller JC Mauro A (eds): The Development and Regenerative Potential of Cardiac Muscle. New York Harwood Academic Publishers 1990 pp 365-384

3. Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice;Steinhelper ME;Am J Physiol,1990

4. Cardiac tumors and dysrhythmias in transgenic mice;Steinhelper ME;Toxicol Pathol,1990

Cited by 60 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3