Affiliation:
1. Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112.
Abstract
BACKGROUND
Studies were carried out to characterize several biochemical features of cultured AT-1 cells.
METHODS AND RESULTS
These cells were obtained from a transplantable atrial cardiomyocyte tumor lineage. Reverse transcriptase-polymerase chain reaction-based analyses demonstrated that the pattern of gene expression of cultured AT-1 cells was similar to that of adult atrial myocytes. AT-1 cells expressed atrial natriuretic factor (ANF), alpha-cardiac myosin heavy chain, alpha-cardiac actin, and connexin43. Radioimmunoassays verified that the cells synthesized, stored, and secreted ANF. Through size-exclusion, reversed-phase, and carboxymethyl-ion-exchange high-performance liquid chromatography, it was shown that cultured AT-1 cells stored ANF as pro-ANF (ANF-[1-126]), which was cosecretionally processed quantitatively to ANF-(1-98) and the bioactive 28-amino-acid ANF-(99-126). In addition, cultured AT-1 cells secreted ANF at almost a sixfold greater rate in response to endothelin-1, a potent secretagogue of ANF. KCl, metenkephalinamide, isoproterenol, phenylephrine, and 12-O-tetradecanoyl-phorbol-13-acetate also stimulated ANF release.
CONCLUSIONS
These studies, in combination with previous findings, demonstrated that cultured AT-1 cells, while maintaining the ability to proliferate, have retained functional, biochemical, and ultrastructural features that are characteristic of adult atrial myocytes.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine
Reference34 articles.
1. Atrial Natriuretic Factor-SV40 T Antigen Transgenes Produce Tumors and Cardiac Arrhythmias in Mice
2. Steinhelper ME Field LT: SV40 large T-antigen induces cardiocyte proliferation in transgenic mice in Oberpriller JO Oberpriller JC Mauro A (eds): The Development and Regenerative Potential of Cardiac Muscle. New York Harwood Academic Publishers 1990 pp 365-384
3. Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice;Steinhelper ME;Am J Physiol,1990
4. Cardiac tumors and dysrhythmias in transgenic mice;Steinhelper ME;Toxicol Pathol,1990
Cited by
60 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献