Circulating MicroRNAs in Delayed Cerebral Infarction After Aneurysmal Subarachnoid Hemorrhage

Abstract

Background Delayed cerebral infarction ( DCI ) is a major cause of morbidities after aneurysmal subarachnoid hemorrhage ( SAH ) and typically starts at day 4 to 7 after initial hemorrhage. Micro RNA s (mi RNAs ) play an important role in posttranscriptional gene expression control, and distinctive patterns of circulating mi RNA changes have been identified for some diseases. We aimed to investigate mi RNA s that characterize SAH patients with DCI compared with those without DCI . Methods and Results Circulating mi RNA s were collected on day 7 after SAH in healthy, SAH ‐free controls (n=20), SAH patients with DCI (n=20), and SAH patients without DCI (n=20). We used the LASSO (least absolute shrinkage and selection operator) method of regression analysis to characterize mi RNA s associated with SAH patients with DCI compared with those without DCI . In the 28 dysregulated mi RNA s associated with DCI and SAH , we found that a combination of 4 mi RNA s (miR‐4532, miR‐4463, miR‐1290, and miR‐4793) could differentiate SAH patients with DCI from those without DCI with an area under the curve of 100% (95% CI 1.000–1.000, P <0.001). This 4‐mi RNA combination could also distinguish SAH patients with or without DCI from healthy controls with areas under the curve of 99.3% (95% CI 0.977–1.000, P <0.001) and 82.0% (95% CI 0.685–0.955, P <0.001), respectively. Conclusions We found a 4‐mi RNA combination that characterized SAH patients with DCI . The findings could guide future mechanistic study to develop therapeutic targets.